HOW TO MAKE A PREPARED SLIDE
If the given sample is not thin and transparent, it cannot be observed by the microscope as the light from the reflector or the light source does not pass tough it. Fibres, pollen, wool, or salt can be observed easily and cover glass is optional. Clear samples are stained first with a drop or two drops of Methylene Blue, Eosin or other dyeing solutions available on the market. (Note These are dyeing solutions and therefore could cause staining of clothing, carpets, and fabrics. Special care should be taken when handling these solutions.)
Also please read the earlier section again "EXPLANATION OF BOTTLES", about the safety information associated with these solutions.)
1) Temporary Mount
Wipe the slide and cover glass clean. Thin sample with a razor blade or the micro-slicer (Note This process should be under an adult's supervision. The blade is very sharp and has to be handled with extreme caution. Please refer to following section for use of micro-slicer). Pick up thinned sample with tweezers and put it on the centre parf of the glass slide. Put one drop of water on the sample with a dissecting needle, or if the sample is dear, use one drop of the above mentioned dyeing solutions (Note The needle has a sharp point so handle with extreme caution) and then gently put on cover glass. Avoid trapping any air bubbles. Remove any excess water or dyeing solution with blotting paper. Now it is ready for observation. (Remember to wash your hands after doing the preparation and remember to dispose of the dyeing solutions according to the instructions given in section "ADVICE FOR SUPERVISING ADULTS")
2) Permanent Mount
Wipe the slide and cover glass clean as above (Temporary Mount). Proceed as above. However before covering the slide with the cover glass add a few drops of gum media (not included in the set available from store) or Canada balsam solution or transparent adhesive glue with a dissecting needle to the slide. Push down on the cover glass with tweezers to fix it in place and leave to dry for about a day.
HOW TO USE THE MICRO-SLICER
Put the specimen which you want to cut for study into the holes of the micro-slicer (Note the micro-slicer contains o sharp edge so handle with extreme caution). Revolve the blade. Then you can get thin slices of the specimen.